实验原理：

      RAP技术通过设计生物素探针组拉取目标RNA，使与其共同作用的RNA或蛋白质（RBPs）富集在磁珠上并被洗脱。最后，通过高通量测序得到该调控RNA转录调控的下游靶基因，同时，也可以通过Western Blot验证RBPs，或通过MS鉴定未知蛋白。

 

产品优势：

1. 伯信独立研发，具有自主知识产权。

2. 灵敏度高，稳定性好。

3. 检测方法领先，结果准确、重复性好。

技术路线：

结果案例：

详见产品说明书：Bes5103-1 RAP-RNA

详见产品说明书：Bes5103-2 RAP-Protein

详见产品说明书：Bes5103-3 RAP-RNA Protein

注：Bes5103-3 RAP-RNA&Protein （同时拉RNA结合RNA和蛋白）的用户可根据实验目的自行参考说明书Bes5103-1 RAP-RNA（只研究RNA结合RNA） 或Bes5103-2 RAP-Protein （只研究RNA结合蛋白）进行实验操作。

文章案例

【2026年】

 

Article：CircROR1 binds HNRNPL to regulate FOXO4 pre-mRNA splicing, promoting cutaneous melanoma metastasis and serving as a therapeutic target via RNAi-loaded PEG-LNPs
Periodicals：Molecular Cancer
IF：33.8990

Article：DNMT2 inhibits anaplastic thyroid cancer progression by downregulating 5’tiRNAGly-GCC production
Periodicals：Cell Death & Disease
IF：9.5998

Article：IGF2BP3 promotes progression of head and neck cancers through the circHECTD2

Periodicals：Molecular Cancer

IF：33.8990

 

Article：N4-acetylcytidine in LncRNA Gm26917 Promotes Translation in Female Germline Stem Cells

Periodicals：Advanced Science

IF：14.1

 

Article：The circular RNA circANK suppresses rice resistance to bacterial blight

Periodicals：The Plant Cell

IF：11.6000

 

Article：The ANXA2P1-hnRNP F-HK2c-Myc Positive Feedback

Periodicals：International Journal of Biological Sciences

IF：9.9995

Article：Super enhancer-driven LINC01013 mediates hypoxia-induced mitochondrial dysfunction by HSPA9 to determine pulmonary arterial smooth muscle cell fate
Periodicals：Cellular and Molecular Life Sciences
IF：6.2001

【2025年】

 

Article：CircROR1 binds HNRNPL to regulate FOXO4 pre-mRNA splicing, promoting cutaneous melanoma metastasis and serving as a therapeutic target via RNAi-loaded PEG-LNPs
Periodicals：Molecular Cancer
IF：33.8990

Article：Targeting cTRIP12 counteracts ferroptosis resistance and augments sensitivity to immunotherapy in pancreatic cancer. 

Periodicals：Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy

IF：15.800

Article：Identification of a JAK–STAT–miR155HG positive feedback loop in regulating natural killer (NK) cells proliferation and effector functions

Periodicals：Acta Pharmaceutica Sinica B

IF：14.700

Article：SenExo-cCCT2 Reprograms Senescence Response and Anti-Tumor Immunity Following FOLFIRINOX Chemotherapy in Pancreatic Ductal Adenocarcinoma.

Periodicals：Advanced Science (Weinheim, Baden-Wurttemburg, Germany)

IF：14.100

Article：CircTP53/USP10/p53 signaling Axis as a Novel Regulator of Progression and Prognosis of Head and Neck Squamous Cell Carcinoma. 

Periodicals：Advanced Science (Weinheim, Baden-Wurttemburg, Germany)

IF：14.100

Article：Cuticle protein mediates the evolution of stress resistance by generating a decoy circular RNA in spider mite.

Periodicals：Science Advances

IF：12.500

Article: N6-methyladnosine of vRNA facilitates influenza A virus replication by promoting the interaction of vRNA with polymerase proteins. 

Periodicals：Proceedings of the National Academy of Sciences of the United States of America

IF：9.100

Article: CSF3R-AS promotes hepatocellular carcinoma progression and sorafenib resistance through the CSF3R/JAK2/STAT3 positive feedback loop.

Periodicals：Cell Death & Disease

IF：8.100

Article：piRNA28846 has the potential to be a novel RNA nucleic acid drug for ovarian cancer.

Periodicals：NPJ Precision Oncology

IF：6.800

【2024年】

Article： Sulindac (K-80003) with nab-paclitaxel and gemcitabine overcomes drug-resistant pancreatic cancer.

Periodicals：Molecular cancer

IF：27.700

Article：Exosomal long non-coding RNA TRPM2-AS promotes angiogenesis in gallbladder cancer through interacting with PABPC1 to activate NOTCH1 signaling pathway.

Periodicals：Molecular cancer

IF：27.700

Article：Escherichia coli-Induced cGLIS3-Mediated Stress Granules Activate the NF-κB Pathway to Promote Intrahepatic Cholangiocarcinoma Progression. 

Periodicals：Advanced Science

IF：14.300

Article：circMIRIAF aggravates myocardial ischemia-reperfusion injury via targeting miR-544/WDR12 axis.

Periodicals：Redox biology

IF：10.700

Article：Blockade of the lncRNA-PART1-PHB2 axis confers resistance to PARP inhibitor and promotes cellular senescence in ovarian cancer.

Periodicals：Cancer Letters

IF：9.100

Article：CircNUP54 promotes hepatocellular carcinoma progression via facilitating HuR cytoplasmic export and stabilizing BIRC3 mRNA.

Periodicals：Cell death & disease

IF：8.100

【2023年】

Article：N6-methyladenosine-modified circRIMS2 mediates synaptic and memory impairments by activating GluN2B ubiquitination in Alzheimer's disease.

Periodicals：Translational Neurodegeneration

IF：12.600

Article：METTL3/YTHDC1-medicated m6A modification of circRNA3634 regulates the proliferation and differentiation of antler chondrocytes by miR-124486-5-MAPK1 axis. 

Periodicals：Cellular & molecular biology letters

IF：9.200

Article：Circular RNA circ0001955 promotes cervical cancer tumorigenesis and metastasis via the miR-188-3p/NCAPG2 axis

Periodicals：Journal of Translational Medicine

IF：7.400

【2022年】

Article：The N6-methyladenosine modifcation of circALG1 promotes the metastasis of colorectal cancer mediated by the miR-342-5p/PGF signalling pathway

Periodicals：Molecular Cancer

IF：37.300

Article：EIF4A3-induced circTOLLIP promotes the progression of hepatocellular carcinoma via the miR-516a-5p/PBX3/EMT pathway

Periodicals：Journal of Experimental & Clinical Cancer Research

IF：11.300

Article：LINC00924-induced fatty acid metabolic reprogramming facilitates gastric cancer peritoneal metastasis via hnRNPC-regulated alternative splicing of Mnk2

Periodicals：Cell Death and Disease

IF：9.000

Article：CircMTO1 suppresses hepatocellular carcinoma progression via the miR-541-5p/ZIC1 axis by regulating Wnt/β-catenin signaling pathway and epithelial-to-mesenchymal transition

Periodicals：Cell Death and Disease

IF：8.469

Article：The pivotal regulatory factor circBRWD1 inhibits arsenic exposure-induced lung cancer occurrence by bindingmRNA and regulating its stability

Periodicals：Molecular Therapy: Oncolytics

IF：6.311